The Kitāb Naʿt al-Ḥayawān is the earliest of a group of illustrated manuscripts dealing with the characteristics of animals and their medicinal uses. The present study considers both the confluence of textual traditions within this work and the stylistic and iconographic relationships of its illustrations, which make it a key witness to early thirteenth-century Arab painting. After a re-evaluation of previous approaches, emphasis is placed on relating image to text, on stylistic affiliations, and on the modalities of production, supported by technical analyses undertaken for the first time. In elucidating the particular context of this unique manuscript, the study contributes to our understanding of a critical period in the development of Middle Eastern painting and art.