Application of plant tissue and cell culture methods has immense potential in the large-scale propagation and conservation of the unexplored biodiversity of plants all over the world and especially in a huge country like India. The present thesis details our efforts in characterization of Glycin max cv PK -472. Plant cell and tissue culture has emerged as major tool in the study of an increasing number of applied and fundamental problems in the plant science and now it has become an important integral constituent of plant biotechnology. Plant cell and culture technology could serve as a supplemented to the conventional methods of plant breeding for production of lite plant lets in vitro which is an important propagation technology to agriculture during the last decade. Significant progress has been made in the propagation of plants. Which novel features through tissue culture technology to meet the ever growing commercial requirements the realization of in vitro multiplication of a large number of clonal plants with the improved charters has been giving significance. Among again legumes soybean (Glycine max L.)