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Monoclonal antibodies are proteins that are developed in the laboratory and can destroy specific targets in the body by binding with them. The targets are usually foreign materials, such as antigens on the surface of cancer cells. The process of monoclonal antibody production begins with the immunization of mice and the isolation of splenocytes. Thereafter, the myeloma cells are fused with isolated splenocytes in the presence of polyethylene glycol (PEG), resulting in the fusion of cell membrane. It further involves clone screening and picking, functional characterization, scaling- up clones producing desired antibodies and weaning-off of selection agents, and expansion of clones producing desired antibodies. The most important applications of monoclonal antibodies include diagnostics, therapeutics, and protein purification. Monoclonal antibodies are used for diagnosing and treating many diseases, including some types of cancer, cardiovascular diseases, deep vein thrombosis, and atherosclerosis. Furthermore, these antibodies can also be used for directly carrying radioactive substances, toxins, and drugs towards the cancer cells. This book outlines the production and application of monoclonal antibodies in detail. It includes contributions of experts and scientists, which will provide innovative insights into the field of pharmaceutical sciences.
Monoclonal antibodies are proteins that are developed in the laboratory and can destroy specific targets in the body by binding with them. The targets are usually foreign materials, such as antigens on the surface of cancer cells. The process of monoclonal antibody production begins with the immunization of mice and the isolation of splenocytes. Thereafter, the myeloma cells are fused with isolated splenocytes in the presence of polyethylene glycol (PEG), resulting in the fusion of cell membrane. It further involves clone screening and picking, functional characterization, scaling- up clones producing desired antibodies and weaning-off of selection agents, and expansion of clones producing desired antibodies. The most important applications of monoclonal antibodies include diagnostics, therapeutics, and protein purification. Monoclonal antibodies are used for diagnosing and treating many diseases, including some types of cancer, cardiovascular diseases, deep vein thrombosis, and atherosclerosis. Furthermore, these antibodies can also be used for directly carrying radioactive substances, toxins, and drugs towards the cancer cells. This book outlines the production and application of monoclonal antibodies in detail. It includes contributions of experts and scientists, which will provide innovative insights into the field of pharmaceutical sciences.
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