This open access book builds on the success of the Second Conference of the Research Project on "Digital Vulnerability in European Private Law", which was held on 16th and 17th May 2024 at the University of Rome Tor Vergata, focusing on the theme "Human Vulnerability in Interaction with AI". Bringing together more than twenty legal scholars and scientists from various fields, including philosophy, sociology, medicine, computer science, the book explores a vast array of topics and applications illustrating the challenges presented by Human Machine Interaction (HMI), and particularly by Artificial Intelligence (AI), to the fluid and multilayered nature of the human condition. In the post European Union AI Act legal landscape, the primary aim of this collection is to test and possibly to boost the distinctiveness of the European position in this regard. Relying on the multidisciplinary composition of the team, the book offers in-depth discussions on important subjects, including, but not limited to: legal subjectivity and individual identity, inclusion/exclusion dynamics arising out of our increasingly digitalized world, scenarios of human-machine hybridization, the descriptive and prescriptive potential of the notion of digital vulnerability to capture individuals& and groups& varying conditions of fragility as well as new avenues of protection for human/fundamental rights in the digitalized world.  The volume helps transformatively reconsider the EU approach to AI risk management and is of enduring value to legal scholars, legal practitioners, and everyone interested in grounding AI in a human-centered and trustworthy legal framework.