This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successfulMethods in Molecular Biologybook series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritativeand practical,Immunometabolism: Methods and Protocolsserves as a vital guide for researchers working at the important interface of immunology and metabolism.