Spatial Omics: Methods for Reconstructing the Spatial Heterogeneity of Biological Tissueis a complete and detailed reference on research methods that combine the in-depth analysis at single cell level with context provided by tissue positioning. The methods covered in the book include Methods that require the mechanical tissue retrieval, digestion and enrichment, Methods based on in situ hybridization and imaging, Methods reliant on next generation sequencing and hybrid techniques, and Computational based methods to determine spatial relatedness of cells. Technologies are focused on the balance between sensitivity, accuracy, and extent of features through which single cells can be evaluated, and more.Cellular and molecular biologists will benefit from the detailed experimental setup information included in this comprehensive book. Computational biologists and computer scientists involved in biological visualization problems can also use this book as a guide to interpret and analyze experimental data. Covers the methods currently available to reconstruct the spatial heterogeneity of biological tissues Provides all methods, including detailed and expansive templates on materials, equipment and steps involved to execute the experiments Authored by researchers from key laboratories around the world who pioneered techniques covered